Abstract
The objective of this study is to establish an in vitro germination and cultivation protocol for murici (Byrsonima cydoniifolia A. Juss.) using zygotic embryos. Therefore, three assays were performed: in assay I, embryo asepsis was tested at exposure times of 5, 10, 15, 20, 25, and 30 minutes in 2.5% sodium hypochlorite, with or without immersion in 70% alcohol; in assay II, MS (MURASHIGE; SKOOG, 1962) e WPM (LLOYD; McCOWN, 1980) culture media were tested at salt concentrations of 25, 50, and 100%, with or without the addition of sucrose, to germinate the buds; in assay III, seedling growth was evaluated in MS and WPM culture media at salt concentrations of 25, 50 and 100%. Sodium hypochlorite (2.5%) with or without 70% alcohol was used to avoid contamination because it was not toxic to murici embryos. Water-agar was the most appropriate culture medium for bud germination, and 50% WPM was appropriate for seedling growth.
Highlights
Murici (Byrsonima ssp.) is a native fruit-producing plant of the Cerrado
All assays were performed in the Plant Tissue Culture Laboratory of the Federal Institute of Goiás (IFGoiano), Rio Verde Campus
The asepsis of B. cydoniifolia embryos should be performed using 70% alcohol for 1 minute followed by the application of sodium hypochlorite (2.5%) for 25 minutes
Summary
It is a dicotyledon that belongs to the Malpighiaceae family. This tree species produces fruits between December and March in the highland regions of Southeastern Brazil, in the Cerrado of Mato Grosso and Goias, and on the coast of North and Northeastern Brazil. The mature fruits are yellow, 1.5 to 2 cm in diameter, have a marked odor, and are characterized as having an exotic flavor and aroma (ALVES; FRANCO, 2003; GUIMARÃES; SILVA, 2008; REZENDE; FRAGA, 2003).The fruits possess a high nutritional value and. Agronomy are mainly consumed fresh or in the form of juice, liquor, jelly, candy, or ice cream (ALMEIDA et al, 1998, 2008; FIGUEIREDO et al, 2005; GUIMARÃES; SILVA, 2008). The local populations that live off of extracting natural resources sell murici in open fairs and local markets (GUSMÃO et al, 2006); there is still no formal market
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