Implication of MMPs in the amplification of the inflammatory response in tissue necrosis induced by Cerastes cerastes venom

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Event Abstract Back to Event Implication of MMPs in the amplification of the inflammatory response in tissue necrosis induced by Cerastes cerastes venom Amina Ami1, Habiba Oussedik-Oumehdi1 and Fatima Laraba-Djebari1* 1 University of Sciences and Technology "Houari Boumediene" - Faculty of Biological Sciences,, Algeria Introduction Besides inducing hemorrhage and tissue necrosis, venom metalloproteinases play a relevant role in the complex and multifactorial inflammatory response characteristic of snake bite envenomation. The endogenous mediators of this inflammatory reaction are likely to contribute significantly to the induced local pathology by taking part in the severity of envenomation. Thereafter, several complications could occur, such as the development of a secondary infection, disability and the progression of necrosis, which can lead to the amputation. The aim of the present work is to determine the possible role of MMPs in amplifying the pathogenesis of acute local tissue damage after viper envenomation. Materials and methods Local venom effects (hemorrhage and necrosis) were induced experimentally by intradermic co-injection of venom and/or the inflammatory exudate. A comparative study was carried out by assessing the hemorrhagic and necrotic spot by the software Wound Area Professional. MMPs expression was determined in skin tissue homogenates by zymography and semi-quantified by Image J 1.46r software. NO levels and MPO activities were also determined in skin homogenates and exudate. Results Results indicated a significant increment in venom induced necrosis and hemorrhage when the venom is co-injected with inflammatory exudate (about 2 folds compared with that observed in the case of the venom alone). Necrotic and hemorrhagic areas were evaluated as 6.9 ± 1.22 cm2 and 11.36 ± 0.91 cm2, respectively. Cerastes cerastes venom induced a prominent inflammatory response characterized by an increase of MPO activity estimated as 3.62 UDO/100 µL for exudate and 0.84 UDO/100 mg for skin homogenates, and also a production of high amounts of NO in exudate (58.06 µM) and in skin homogenates (12.01µM). Considerable production of MMPs was observed, including MMP-2 in the active form and MMP-9 in the latent form. Conclusion Unlike neutrophils and NO, MMPs seemed to play a prominent role in the acute local pathological alterations induced by Cerastes cerastes venom in this experimental model. MMPs contribution in local tissue necrosis raises important therapeutic issues regarding the treatment of local envenoming. Keywords: Cerastes cerastes, Hemorrhage, Inflammatory exudate, Matrix métalloprotéinases, Necrosis, venom Conference: The First International Congress of Immunology and Molecular Immunopathology (CIMIP2014), Tlemcen, Algeria, 17 Oct - 20 Oct, 2014. Presentation Type: Poster Presentation Topic: Immunity – Nutrition – Cancer Citation: Ami A, Oussedik-Oumehdi H and Laraba-Djebari F (2014). Implication of MMPs in the amplification of the inflammatory response in tissue necrosis induced by Cerastes cerastes venom. Front. Immunol. Conference Abstract: The First International Congress of Immunology and Molecular Immunopathology (CIMIP2014). doi: 10.3389/conf.fimmu.2014.04.00003 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 08 Sep 2014; Published Online: 01 Dec 2014. * Correspondence: Prof. Fatima Laraba-Djebari, University of Sciences and Technology "Houari Boumediene" - Faculty of Biological Sciences,, Algiers, 16 000, Algeria, flaraba@hotmail.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Amina Ami Habiba Oussedik-Oumehdi Fatima Laraba-Djebari Google Amina Ami Habiba Oussedik-Oumehdi Fatima Laraba-Djebari Google Scholar Amina Ami Habiba Oussedik-Oumehdi Fatima Laraba-Djebari PubMed Amina Ami Habiba Oussedik-Oumehdi Fatima Laraba-Djebari Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.