Involvement of histamine H2-receptors in the induced pulmonary inflammation by Androctonus australis hector venom

Abstract

Listen

Translate article

Event Abstract Back to Event Involvement of histamine H2-receptors in the induced pulmonary inflammation by Androctonus australis hector venom Amal Lamraoui1, Sonia Adi-Bessalem1 and Fatima Laraba-Djebari1* 1 University of Sciences and Technology "Houari Boumediene", Faculty of Biological Sciences, Department Cellular and Molecular Biology, Algeria Abstract Introduction: Acute lung inflammation is an important feature of scorpion envenoming syndrome which can lead to edema forming that is usually fatal. However, the mechanism of the triggering of this response still poorly understood. Materials and methods: The present study highlights the role of histamine H2-receptors in the activation of the pulmonary inflammatory response and edema forming induced by Androctonus australis hector (Aah) scorpion venom, by evaluating the effect of an antagonist of histamine H2-receptor on lung inflammatory response assessed after injection of subletal dose of Aah venom into mice. The lung tissue inflammation was evaluated by an analyze of pulmonary parenchyma structure, vascular permeability changes, cellular peroxidase activities including eosinophil peroxidase (EPO) and myeloperoxidase (MPO) evaluatioan and by assessing the oxidative stress markers (malondialdehyde (MDA) and catalase). Results: Obtained results showed that Aah venom induced an increase in the pulmonary vascular permeability with an infiltration of neutrophil and eosinophil cells into the lung tissue and displayed imbalanced redox status with decreased catalase and increased MDA. These results are correlated with the disorganization of the lung parenchyma marked by the thickening of the inter-alveolar septa, the presence of inflammatory cells, and also with hemorrhages and edema forming. Pretreatment with H2-receptor antagonist reduced the pulmonary vascular permeability, MPO and EPO activities. Moreover, this pretreatment was able to decrease the MDA level and increase the catalase activity. The microscopic analysis revealed a reduction in the thickening of inter-alveolar septa and an absence of hemorrhagic areas. Nevertheless, the persistence of a few inflammatory cells was observed. Discussion: The acute pulmonary inflammation induced by Aah venom results from the intervention of several related mechanisms. The present findings show that histamine by binding with its H2-receptors is involved in this induced response. Conclusion: Targeting histamine H2-receptors using their antagonist drugs could be beneficial in treating envenomed patients. Keywords: Histamine H2-receptors, Lung, Inflammation, Oxidative Stress, scorpion venom. Conference: The First International Congress of Immunology and Molecular Immunopathology (CIMIP2014), Tlemcen, Algeria, 17 Oct - 20 Oct, 2014. Presentation Type: Poster Presentation Topic: Immunity – Nutrition – Cancer Citation: Lamraoui A, Adi-Bessalem S and Laraba-Djebari F (2014). Involvement of histamine H2-receptors in the induced pulmonary inflammation by Androctonus australis hector venom. Front. Immunol. Conference Abstract: The First International Congress of Immunology and Molecular Immunopathology (CIMIP2014). doi: 10.3389/conf.fimmu.2014.04.00009 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 09 Sep 2014; Published Online: 01 Dec 2014. * Correspondence: Prof. Fatima Laraba-Djebari, University of Sciences and Technology "Houari Boumediene", Faculty of Biological Sciences, Department Cellular and Molecular Biology, Algiers, 16111, Algeria, flaraba@hotmail.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Amal Lamraoui Sonia Adi-Bessalem Fatima Laraba-Djebari Google Amal Lamraoui Sonia Adi-Bessalem Fatima Laraba-Djebari Google Scholar Amal Lamraoui Sonia Adi-Bessalem Fatima Laraba-Djebari PubMed Amal Lamraoui Sonia Adi-Bessalem Fatima Laraba-Djebari Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.