Modulation of nitric oxide production by peripheral blood mononuclear cells of Algerian patients with Alzheimer’s disease using vitamin A and vitamin D; a therapeutic approach

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Event Abstract Back to Event Modulation of nitric oxide production by peripheral blood mononuclear cells of Algerian patients with Alzheimer’s disease using vitamin A and vitamin D; a therapeutic approach Nassima Behairi1*, Mourad Belkhelfa1, Soreya Belarbi2, Meriem Tazir2 and Chafia Touil-Boukoffa1 1 University of Sciences and Technology Houari Boumediene (USTHB), Department of Molecular and Cellular Biology, Faculty of Biological Sciences, Algeria 2 Mustapha Pacha Hospital, Department of Neurology, Algeria Abstract: Introduction: Alzheimer’s disease (AD) constitutes a progressive neurodegenerative disorder and the main cause of dementia in the elderly. A growing body of evidence has associated neuroinflammation and oxidative stress to the onset and the progression of the pathology. Inducible Nitric Oxide Synthase (iNOS) and its product (NO) have been involved in AD pathogenesis. Antioxidant and immune-modulatory properties of vitamin A and vitamin D have been shown in several immune disorders. In our current study, we investigate the modulatory effects of both vitamin A and vitamin D on nitric oxide production in Algerian patients with Alzheimer’s disease. Materials and methods: Peripheral Blood Mononuclear Cells (PBMCs) were isolated from 15 Algerian patients with Alzheimer’s disease. Ex vivo PBMCs cultures were conducted in presence of vitamin A or vitamin D in RPMI 1640 medium for 24 hours. Nitric oxide production in culture supernatants was assessed using modified Griess method. Results: Both vitamin A and vitamin D down-modulated the nitric oxide production by PBMCs of Alzheimer’s disease patients. Interestingly, the vitamin D effect was more important compared to vitamin A one. Discussion/Conclusion: Our reports suggest that modulatory effects of vitamin A and vitamin D on nitric oxide production can be used as a promising therapeutic approach in Alzheimer’s disease. Acknowledgements The author thanks Dr. Hayet Rafaa(Cytokines and NO-Synthase; immunity and pathogeny team, USTHB, Algiers, Algeria), Dr. Islem Kediha (Department of Neurology, Mustapha Pacha Hospital, Algiers, Algeria) and Mr. Lyes Achouri for their help and all the patients who have participated to this study and their families. Keywords: Alzheimer’s disease, Nitric Oxide, peripheral blood mononuclear cells, Vitamin A, Vitamin D Conference: The First International Congress of Immunology and Molecular Immunopathology (CIMIP2014), Tlemcen, Algeria, 17 Oct - 20 Oct, 2014. Presentation Type: Poster Presentation Topic: Immunity – Nutrition – Cancer Citation: Behairi N, Belkhelfa M, Belarbi S, Tazir M and Touil-Boukoffa C (2014). Modulation of nitric oxide production by peripheral blood mononuclear cells of Algerian patients with Alzheimer’s disease using vitamin A and vitamin D; a therapeutic approach. Front. Immunol. Conference Abstract: The First International Congress of Immunology and Molecular Immunopathology (CIMIP2014). doi: 10.3389/conf.fimmu.2014.04.00013 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 11 Sep 2014; Published Online: 01 Dec 2014. * Correspondence: Miss. Nassima Behairi, University of Sciences and Technology Houari Boumediene (USTHB), Department of Molecular and Cellular Biology, Faculty of Biological Sciences, Algiers, 16111, Algeria, behairi_nassima@hotmail.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Nassima Behairi Mourad Belkhelfa Soreya Belarbi Meriem Tazir Chafia Touil-Boukoffa Google Nassima Behairi Mourad Belkhelfa Soreya Belarbi Meriem Tazir Chafia Touil-Boukoffa Google Scholar Nassima Behairi Mourad Belkhelfa Soreya Belarbi Meriem Tazir Chafia Touil-Boukoffa PubMed Nassima Behairi Mourad Belkhelfa Soreya Belarbi Meriem Tazir Chafia Touil-Boukoffa Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.