Abstract
The specific activity of nearly all enzymes of the carbohydrate metabolism declines during aging. In rat lenses, this decrease is accompanied by a loss in the heat-unstable portions of the aldolase isoenzymes A and C. By means of the quantitative immunologic titration and the use of enzymespecific antibodies, inactive aldolase molecules, the so-called cross-reacting material, could be determined in 2-year-old rat lenses. The cross-reacting material could also be determined for phosphofructokinase in the nucleus of 6-year-old rabbit lenses.
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