Hyper xylanase production and potential of xylooligosaccharides formation from a novel Bacillus australimaris KS2

Abstract

The study has been designed to isolate xylanase-producing bacteria and parametric optimization for maximum production. The xylanase was utilized for enzymatic hydrolysis of wheat bran (WB) and wheat bran extracted xylan (WBEx) to obtain xylooligosaccharides. The research was planned for value addition in the agriculture sector with low-cost enzyme production and its utilization in prebiotic production from agro-residue. The xylanase obtained from the new isolate Bacillus australimaris KS2 was optimized with 2.0% of 24 h inoculum supplemented with 2.0% wheat bran at pH 7, 35 °C for 48 h with an agitation rate of 150 rpm. There was a 2.96-fold increase in xylanase activity from its initial (2997.07 IU/mL) activity to the optimized condition (8873.07 IU/mL). The maximum reducing sugar production over enzymatically hydrolyzed WB was found after 8 h of reaction time at pH 5, 55 °C with 375 units of enzyme. In the case of enzymatically hydrolyzed WBEx, the maximum production of 588 mg/g was achieved after 16 h at pH 6 and 55 °C of reaction temperature at the same amount of enzyme dose. The results demonstrated that the new isolate Bacillus australimaris KS2 was an efficient xylanase producer. Xylooligosaccharides produced through enzymatic hydrolysis of WBEx showed good prebiotic potential over Lactobacillus plantarum, Lactobacillus brevis, and Lactobacillus rhamnosus.