Optimization of Xylanase production from Penicillium sp.WX-Z1 by a two-step statistical strategy: Plackett-Burman and Box-Behnken experimental design.

Fengjie Cui,Liming Zhao

doi:10.3390/ijms130810630

Abstract

The objective of the study was to optimize the nutrition sources in a culture medium for the production of xylanase from Penicillium sp.WX-Z1 using Plackett-Burman design and Box-Behnken design. The Plackett-Burman multifactorial design was first employed to screen the important nutrient sources in the medium for xylanase production by Penicillium sp.WX-Z1 and subsequent use of the response surface methodology (RSM) was further optimized for xylanase production by Box-Behnken design. The important nutrient sources in the culture medium, identified by the initial screening method of Placket-Burman, were wheat bran, yeast extract, NaNO3, MgSO4, and CaCl2. The optimal amounts (in g/L) for maximum production of xylanase were: wheat bran, 32.8; yeast extract, 1.02; NaNO3, 12.71; MgSO4, 0.96; and CaCl2, 1.04. Using this statistical experimental design, the xylanase production under optimal condition reached 46.50 U/mL and an increase in xylanase activity of 1.34-fold was obtained compared with the original medium for fermentation carried out in a 30-L bioreactor.

Highlights

Xylanases have been widely used for clarifying fruit juices and wine [1,2,3], in food processing in combination with cellulases [4,5], bleaching in the paper and pulp industry [6,7,8], and hydrolyzing agricultural waste to produce renewable energy products in the biofuel industry [9,10]
Sodium nitrate and yeast extract resulted in higher xylanase production by Penicillium oxalicum in comparison to other nitrogen sources [12]
The nutrient sources were reduced to five major variables by the Plackett-Burman experimental design, suggesting that Plackett-Burman design is a powerful tool for screening fermentation factors

Summary

Introduction

Xylanases have been widely used for clarifying fruit juices and wine [1,2,3], in food processing in combination with cellulases [4,5], bleaching in the paper and pulp industry [6,7,8], and hydrolyzing agricultural waste to produce renewable energy products in the biofuel industry [9,10]. The production of xylanase based on microbial fermentation and biosynthesis renders its industrial application more feasible and economical. Filamentous fungi have been widely used to produce hydrolytic enzymes for industrial application, including xylanases, whose levels in fungi are generally much higher than in yeast and bacteria. The most common industrial xylanase producing strains are the species Aspergillus sp. Sodium nitrate and yeast extract resulted in higher xylanase production by Penicillium oxalicum in comparison to other nitrogen sources [12]. Some other important nitrogen sources such as trypton and casein were not investigated in their research. Amino acids and their analogues are known to simulate the production of enzymes, such as α-amylase, β-galactosidase and xylanase. The production cost for xylanase should be significantly reduced

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Conclusion