High-Quality Draft Genome Sequence of Francisella tularensis subsp. holarctica Strain 08T0073 Isolated from a Wild European Hare.

Anne Busch,Mats Forsman,Kerstin Myrtennäs,Herbert Tomaso,Martin Runge,Prasad Thomas,Silke Braune

doi:10.1128/genomea.01577-16

Abstract

ABSTRACTHere, we report a high-quality draft genome sequence of Francisella tularensis subsp. holarctica strain 08T0073, isolated from the cadaver of a wild European hare (Lepus europaeus) found near Helmstedt, Lower Saxony, Germany, in 2007. In Germany, infected hares are a major source of tularemia in humans.

Highlights

We report a high-quality draft genome sequence of Francisella tularensis subsp. holarctica strain 08T0073, isolated from the cadaver of a wild European hare (Lepus europaeus) found near Helmstedt, Lower Saxony, Germany, in 2007
We describe the genome sequence of F. tularensis subsp. holarctica strain 08T0073, which caused fatal tularemia in a hare
Bacterial cells were harvested after 72 h by centrifugation, and the DNA was purified using QIAGEN Genomic-tip 20/G and a QIAGEN Genomic DNA buffer set kit (Qiagen, Hilden, Germany)

Summary

Introduction

We report a high-quality draft genome sequence of Francisella tularensis subsp. holarctica strain 08T0073, isolated from the cadaver of a wild European hare (Lepus europaeus) found near Helmstedt, Lower Saxony, Germany, in 2007. We describe the genome sequence of F. tularensis subsp. Holarctica strain 08T0073 ( known as FDC386 and W2990), which caused fatal tularemia in a hare. The phylogeny of the strain was further elucidated using real-time PCR assays and was characterized as genetic clade B.12, subclade B.33/34/75.

Results

Conclusion