Abstract

Desulfovibrio carbinoliphilus subsp. oakridgensis FW-101-2B is an anaerobic, organic acid/alcohol-oxidizing, sulfate-reducing δ-proteobacterium. FW-101-2B was isolated from contaminated groundwater at The Field Research Center at Oak Ridge National Lab after in situ stimulation for heavy metal-reducing conditions. The genome will help elucidate the metabolic potential of sulfate-reducing bacteria during uranium reduction.

Highlights

  • Desulfovibrio carbinoliphilus subsp. oakridgensis FW-101-2B was isolated from groundwater of well FW-101 at The Field Research Center (FRC) at Oak Ridge National Lab (ORNL)

  • Groundwater from well FW-101 at the FRC site was collected during the uranium-reduction phase of a previously described biostimulation experiment [3] and used as inoculum for an enrichment culture for sulfate-reducing bacteria

  • Sequence determination revealed a 4.1-Mb genome with 66.5% GϩC content, which is comparable to D. carbinoliphilus (63% GϩC) and Desulfovibrio vulgaris (63.3% GϩC)

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Summary

Introduction

Desulfovibrio carbinoliphilus subsp. oakridgensis FW-101-2B was isolated from groundwater of well FW-101 at The Field Research Center (FRC) at Oak Ridge National Lab (ORNL). Oakridgensis FW-101-2B was isolated from groundwater of well FW-101 at The Field Research Center (FRC) at Oak Ridge National Lab (ORNL). ICP-MS analysis of FW-101 groundwater in late 2001 estimated uranium concentrations between 20 and 250 ppm, and chromium concentrations between 35 and 85 ppm. Previous studies have demonstrated reduction of nitrate and uranium levels in the subsurface upon bio-stimulation with ethanol at the FRC [1,2,3].

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Conclusion

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