Abstract
Indian Journal of Natural Products,2022,36,1,24-27.DOI:10.5530/ijnp.2022.1.4Published:February 2023Type:Original ArticleAuthors:Ravichand Meshram, Umakant Sahu, Arun K S Parihar, Prashant K Gupta, and Nagendra Singh Chauhan Author(s) affiliations:Ravichand Meshram1, Umakant Sahu2, Arun K S Parihar2, Prashant K Gupta1,3, Nagendra Singh Chauhan2,* 1Shri Narayan Prasad Awasthi Government Ayurved College, Raipur, Chhattisgarh, INDIA. 2Drugs Testing Laboratory Avam Anusandhan Kendra, G.E. Road, Raipur, Chhattisgarh, INDIA. 3Department of Kaumarabhritya, All India Institute of Ayurveda, New Delhi, INDIA. Abstract:The aim of this study was to find out a simple, accurate and sensitive HPTLC method for the detection and quantification of marker molecule Gallic and Piperine in Padmakadi churna for standardization. Ethanolic extraction was performed for analysis. HPTLC was done using gallic acid and Piperine as a standard. The mobile phase was a mixture of Toulene:ethyl acetate:Ethanol:Formic acid (6:3:1:0.3 v/v/v) and detection at 254 nm. The Rf was detected at - 0.39 for Gallic acid and Rf - 0.55 for Piperine was identified in Padmakadi Churna. The simple and sensitive HPTLC method was successfully developed for determination of Gallic acid and Piperine in Padmakadi Churna. The proposed HPTLC method was found to be simple, fast and inexpensive, and can be used for the routine quality control of raw materials. Keywords:Gallic acid, HPTLC, Padmakadi Churna, Physiochemical, Piperine, Standardization.View:PDF (510.58 KB)
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