HETEROTRANSPLANTATIONS AND GENOMIC IN CANCER RESEARCH

doi:10.46545/aijcs.v3i1.220

Abstract

We performed ,in 1975, the first heterotransplantation of invertebrate A.O in nude mouse, then a double heterotransplantation of human tumor and Axial organ next to this last one, always in nude mouse: The human tumor was rejected in 50% of observed cases. Some years later, we found that A.O cells exerted an induced and spontaneous cytotoxicity against SP2 and MBL2 mouse tumoral cells. Recently, we discovered a sea star Igkappa gene with immune properties. This gene was inserted in a CMV(cytomegalovirus) and finally in a plasmid called « young » plasmid. The induced« young » protein exerted a spontaneous cytotoxicity against osteosarcom cells (U2oS cells) against A-375 melanome cells and Hela cells

Highlights

In 1975, Leclerc transplanted with success an invertebrate organ: the sea star axial organ(a primitive lymphoïd organ) in nude mouse (1) risked a double heterotransplantation always in nude mouse: it was composed of a sea star axial organone and a human tumor skin one besides the precedent one, under the skin of nude mouse, in back position.(Unpublished results) In 1983, Luquet and Leclerc (2) shown that the axial organ cells (AO cells), exerted a spontaneous and induced cytotoxicity against mouse SP2 myeloma cells and MBL2 cells
40 years later, we discovered a sea star Igkappa gene (3), with immune properties (4) We have studied the behaviour of the « young » protein secreted by the sea star Igkappa gene, in front of human malignant :A-375 melanome cells, human Osteosarcome cells (U2oS cells) and human malignant cells Hela, by the use of plasmids
Animals a)Asterias rubens, a sea star was collected at Arcachon (France)/ the A.O was excised with pinces and passed or not in antibiotics according to the used method. b)Nude mice were purchased by the CSEAL-CNRS Orléans.-La source c)Mouse SP2 and MBL2 cells were cultured in our laboratory d)Gene cloning in a cytomegalovirus (CMV) was done as seen in( figures 1 and 2), from the sea star Igkappa gene(3).It constitutes the « promoter »

Summary

Introduction

INTRODUCTION In 1975, Leclerc transplanted with success an invertebrate organ: the sea star axial organ(a primitive lymphoïd organ) in nude mouse (1) risked a double heterotransplantation always in nude mouse: it was composed of a sea star axial organone and a human tumor skin one besides the precedent one, under the skin of nude mouse, in back position.(Unpublished results) In 1983, Luquet and Leclerc (2) shown that the axial organ cells (AO cells), exerted a spontaneous and induced cytotoxicity against mouse SP2 myeloma cells and MBL2 cells. 40 years later, we discovered a sea star Igkappa gene (3), with immune properties (4) We have studied the behaviour of the « young » protein secreted by the sea star Igkappa gene, in front of human malignant :A-375 melanome cells, human Osteosarcome cells (U2oS cells) and human malignant cells Hela, by the use of plasmids .

Results

Conclusion