Abstract
ABSTRACTFaustoviruses are amoeba-infecting giant viruses closely related to the Asfarviridae family. Here, we report the isolation, genome sequencing, and annotation of ST1 and LC9, two new strains belonging to lineages L and E9 of faustoviruses, currently represented by only one representative each.
Highlights
Faustoviruses are amoeba-infecting giant viruses closely related to the Asfarviridae family
For functional annotation, predicted proteins were searched for conserved domains and putative functions using the Pfam protein families database [10] and NCBI Conserved Domain Database search tools [11]
The five scaffolds comprising the faustovirus LC9 genome have a total size of 470,227 bp and a mean GϩC content of 39.8%
Summary
Faustoviruses are amoeba-infecting giant viruses closely related to the Asfarviridae family. ST1 was isolated following the procedure previously described by Bou Khalil et al [4]. It was produced and purified for genome sequencing on the Illumina MiSeq platform with a 2 ϫ 250-bp library in paired-end, parallel format. The genome was assembled with the Abyss program [5] and joined into a single scaffold with SSPACE [6].
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