Abstract
ABSTRACTWe report here the high-quality genome sequences of three Streptomyces spp. isolated as part of a long-term study of microbial soil ecology. Streptomyces sp. strain GS93-23 was isolated from naturally disease-suppressive soil (DSS) in Grand Rapids, MN, and Streptomyces sp. strains S3-4 and 3211-3 were isolated from experimental plots in the Cedar Creek Ecosystem Science Reserve (CCESR).
Highlights
We report here the high-quality genome sequences of three Streptomyces spp. isolated as part of a long-term study of microbial soil ecology
Subread filtering from three single-molecule real-time (SMRT) cells for each genome yielded approximately 1 Gb of sequence information for each genome (100ϫ coverage)
Contig assembly was performed with HGAP version 3 [9], with default parameters, in the PacBio SMRT Analysis Portal version 2.3
Summary
We report here the high-quality genome sequences of three Streptomyces spp. isolated as part of a long-term study of microbial soil ecology. Each genome was initially sequenced and assembled from long reads using a Pacific Biosciences single-molecule sequencer at the Mayo Clinic (Rochester, MN). Subread filtering from three single-molecule real-time (SMRT) cells for each genome (two P4-C2 chemistry plus one P6-C4 chemistry) yielded approximately 1 Gb of sequence information for each genome (100ϫ coverage). Contig assembly was performed with HGAP version 3 [9], with default parameters, in the PacBio SMRT Analysis Portal version 2.3.
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