Abstract
ABSTRACTThe self-flocculation of yeast cells presents advantages for continuous ethanol fermentation such as their self-immobilization within fermenters for high density to improve ethanol productivity and cost-effective biomass recovery by gravity sedimentation. We sequenced and analyzed the genome of the self-flocculating Saccharomyces cerevisiae SPSC01 for the industrial production of fuel ethanol.
Highlights
The self-flocculation of yeast cells presents advantages for continuous ethanol fermentation such as their self-immobilization within fermenters for high density to improve ethanol productivity and cost-effective biomass recovery by gravity sedimentation
Different mutation types and positions of Single nucleotide polymorphisms (SNPs) in the coding sequence, intergenic regions, and introns were classified quantitatively, through which 50,165 SNPs (62.4%) were identified in the coding regions and 30,223 SNPs (37.6%) in the noncoding regions
A phylogenetic tree of SPSC01 was constructed in comparison with published genome sequences of yeast strains, which demonstrated that SPSC01 is close to S. cerevisiae Kyokai no. 7, which is used for fermentation to produce sake in Japan [16]
Summary
The self-flocculation of yeast cells presents advantages for continuous ethanol fermentation such as their self-immobilization within fermenters for high density to improve ethanol productivity and cost-effective biomass recovery by gravity sedimentation. The SPSC01 genome is ϳ15 Mb, encompassing a capacity for 5,315 genes with an average length of 1,532 bp.
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