Abstract
ABSTRACTSporomusa acidovorans DSM 3132 is a strictly anaerobic, spore-forming and acetogenic bacterium, which was isolated from effluent of an alcohol distillation fermenter. The genome harbors genes involved in the Wood-Ljungdahl pathway for carbon fixation and several genes for glycerol metabolism. The genome (6.06 Mb) contains 4,506 predicted protein-encoding genes.
Highlights
Sporomusa acidovorans DSM 3132 is a strictly anaerobic, spore-forming and acetogenic bacterium, which was isolated from effluent of an alcohol distillation fermenter
The draft genome of S. acidovorans DSM 3132 consisted of a single chromosome (6,060,615 bp) with an overall GϩC content of 44.58%
The draft genome contained 10 rRNA genes, 99 tRNA genes, 4,506 protein-encoding genes with predicted functions, and 1,263 genes coding for hypothetical proteins
Summary
Sporomusa acidovorans DSM 3132 is a strictly anaerobic, spore-forming and acetogenic bacterium, which was isolated from effluent of an alcohol distillation fermenter. Chromosomal DNA of S. acidovorans DSM 3132 was isolated using the MasterPure complete DNA purification kit as recommended by the manufacturer (Epicentre, Madison, WI, USA). The extracted DNA was used to generate Illumina shotgun paired-end sequencing libraries, which were sequenced with a MiSeq instrument and the MiSeq reagent kit version 3, as recommended by the manufacturer (Illumina, San Diego, CA, USA). Quality filtering using Trimmomatic version 0.32 [9] resulted in 2,367,326 pairedend reads.
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