Abstract
We used paired-end Illumina deep sequencing and de novo assembly to determine the genome sequence of herpes simplex virus 1 (HSV-1) strain MacIntyre (aka McIntyre). The MacIntyre strain originated from the brain of a patient with lethal HSV encephalitis and has a unique limitation in its neuronal spread, moving solely in the retrograde direction.
Highlights
We used Illumina deep sequencing and de novo assembly to determine the genome sequence of herpes simplex virus 1 (HSV-1) strain MacIntyre
The MacIntyre strain originated from the brain of a patient with lethal HSV encephalitis [1]
As in prior studies [17, 24,25,26,27,28,29], we found that a majority of HSV MacIntyre proteins have coding variations compared to those of the HSV-1 reference strain 17
Summary
We used Illumina deep sequencing and de novo assembly to determine the genome sequence of herpes simplex virus 1 (HSV-1) strain MacIntyre ( spelled MacIntyre B, McIntyre, and McIntyre B). The MacIntyre strain originated from the brain of a patient with lethal HSV encephalitis [1]. MacIntyre was passaged through multiple cell types and species [1, 2]. Later studies revealed a severe defect in the anterograde spread of HSV-1 MacIntyre in the central nervous systems in rat, mouse, and primate models [4,5,6,7,8,9,10,11,12].
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