Abstract
Bacillus ligninesis strain L1, isolated from seafloor sediment, was able to grow on medium with lignin as its sole carbon source. Here, we report a 3.8-Mbp high-quality genome sequence for this bacterium. The genes involving ectoine and glycine betaine synthesis, as well as those involved in the degradation of lignin, were identified.
Highlights
Bacillus ligninesis strain L1, isolated from seafloor sediment, was able to grow on medium with lignin as its sole carbon source
A total of ~833 Mb of paired reads with about 213-fold coverage of the genome was generated, which was trimmed to ~439 Mb of paired reads and assembled into 241 contigs and 72 scaffolds using the Short Oligonucleotide Alignment Program (SOAP)denovo software [1]
The open reading frames (ORFs) were identified with the GeneMark gene prediction tool and further analyzed using BLAST to screen the nonredundant protein database, and the relevant gene functions were determined with the help of the Clusters of Orthologous Genes (COGs) [2] and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases
Summary
Bacillus ligninesis strain L1, isolated from seafloor sediment, was able to grow on medium with lignin as its sole carbon source. Strain L1 is able to utilize lignin as its sole carbon source, which presents its potential value in the cellulosic biofuels industry. The whole genome sequence of B. ligninesis L1 was obtained using the Illumina/Solexa HiSeq 2000 sequencing system at Majorbio BioTech (China) with a paired-end library.
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