Abstract
ABSTRACTRhizobium esperanzae CNPSo 668T is a nitrogen-fixing symbiont of Phaseolus vulgaris isolated from Mexican soils. Its genome is estimated at 6,294,057 bp, with 6,219 coding sequences (CDSs) showing higher similarity (92.9%) with Rhizobium etli. Three copies of the regulatory nodD, in addition to other nodulation genes, should define its host specificity.
Highlights
Rhizobium esperanzae CNPSo 668T is a nitrogen-fixing symbiont of Phaseolus vulgaris isolated from Mexican soils
Sequences were submitted to RAST [6], and the annotation identified 6,219 coding sequences (CDSs), with 43% classified in 467 subsystems
Other interesting categories included 86 CDSs related to virulence, disease, and defense, most (76%) of which involved resistance to antibiotics and toxic compounds, 172 CDSs classified in a variety of stress response categories, and 135 CDSs for motility and chemotaxis
Summary
Rhizobium esperanzae CNPSo 668T is a nitrogen-fixing symbiont of Phaseolus vulgaris isolated from Mexican soils. Total DNA was extracted using the DNeasy blood and tissue kit (Qiagen) and processed on the MiSeq platform (Illumina) at Embrapa Soja. Paired-end reads obtained by shotgun sequencing allowed a genome coverage of 21-fold. The genome was assembled by the A5-miseq pipeline (de novo assembly) [5] and estimated at 6,294,057 bp, with 77 contigs and a GϩC content of 61.1 mol%.
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