Abstract
Momo is a newly discovered phage of Mycobacterium smegmatis mc2155. Momo has a double-stranded DNA genome 154,553 bp in length, with 233 predicted protein-encoding genes, 34 tRNA genes, and one transfer-messenger RNA (tmRNA) gene. Momo has a myoviral morphology and shares extensive nucleotide sequence similarity with subcluster C1 mycobacteriophages.
Highlights
Momo is a newly discovered phage of Mycobacterium smegmatis mc2155
Mycobacteriophage Momo was isolated by SEA-PHAGES students from a soil sample collected in Pittsburgh, Pennsylvania, by direct plating on Mycobacterium smegmatis mc2155
Comparison of Momo with other sequenced phages showed that it is closely related to mycobacteriophages in cluster C, which includes the generalized transducing phage Bxz1 [4, 5]
Summary
Momo is a newly discovered phage of Mycobacterium smegmatis mc2155. Momo has a double-stranded DNA genome 154,553 bp in length, with 233 predicted protein-encoding genes, 34 tRNA genes, and one transfer-messenger RNA (tmRNA) gene. Mycobacteriophages provide insights into viral diversity and evolution as well as facilitating development of tools for mycobacterial genetics [1]. Exploration of viral diversity is facilitated by the Science Education Alliance Phage Hunters Advancing Genomics and Evolutionary Science (SEA-PHAGES) program, in which undergraduate students discover and characterize novel bacteriophages [2]. Mycobacteriophage Momo was isolated by SEA-PHAGES students from a soil sample collected in Pittsburgh, Pennsylvania, by direct plating on Mycobacterium smegmatis mc2155.
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