Abstract
Halomonas sp. strain KO116 was isolated from Nile Delta Mediterranean Sea surface water enriched with insoluble organosolv lignin. It was further screened for growth on alkali lignin minimal salts medium agar. The strain tolerates the ionic liquid 1-ethyl-3-methylimidazolium acetate. Its complete genome sequence is presented in this report.
Highlights
Lignin, both a major organic compound of plants and a waste product of industrial processes, is a suitable candidate for the production of biofuels because of its high energy content and abundance in nature (1)
Halomonas sp. strain KO116 was isolated from surface seawater from the Nile Delta Mediterranean Sea and enriched with 0.05% organosolv lignin, 0.05% 1-ethyl-3-methylimidazodium acetate, and 0.0015 M phosphate, and plated onto minimal medium (ONR7a, DSMZ medium 950) agar containing 0.05% alkali lignin
The DNA was sequenced by paired-end Illumina MiSeq (University of Tennessee) and PacBio RSII systems (University of Maryland, Institute for Genome Sciences) to generate 400ϫ and 170ϫ coverages, respectively
Summary
Both a major organic compound of plants and a waste product of industrial processes, is a suitable candidate for the production of biofuels because of its high energy content and abundance in nature (1). The pretreatment of the lignin with ionic liquid reduces the total energy required in the bioconversion process, but its negative effect on enzyme activity makes it a limiting factor in the search for highly efficient lignin degraders (3). Strain KO116 was isolated from surface seawater from the Nile Delta Mediterranean Sea and enriched with 0.05% organosolv lignin, 0.05% 1-ethyl-3-methylimidazodium acetate, and 0.0015 M phosphate, and plated onto minimal medium (ONR7a, DSMZ medium 950) agar containing 0.05% (wt/vol) alkali lignin.
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