Abstract
ABSTRACTHere, we report the first sequenced genome of an indigenous Australian wine isolate of Torulaspora delbrueckii using the Oxford Nanopore MinION and Illumina HiSeq sequencing platforms. The genome size is 9.4 Mb and contains 4,831 genes.
Highlights
We report the first sequenced genome of an indigenous Australian wine isolate of Torulaspora delbrueckii using the Oxford Nanopore MinION and Illumina HiSeq sequencing platforms
T. delbrueckii strain COFT1 was isolated from a spontaneous wine fermentation at the Yalumba Wine Company (Angaston, South Australia, Australia)
Passed reads were trimmed for adapters using PoreChop version 0.2.3 and assembled using SMARTdenovo version 1.0
Summary
We report the first sequenced genome of an indigenous Australian wine isolate of Torulaspora delbrueckii using the Oxford Nanopore MinION and Illumina HiSeq sequencing platforms. T. delbrueckii strain COFT1 was isolated from a spontaneous wine fermentation at the Yalumba Wine Company (Angaston, South Australia, Australia). High-molecularweight genomic DNA was extracted according to the phenol-chloroform protocol [4], including a 2-h preincubation with Zymolase. DNA was prepared for sequencing with the MinION device using the SQK-LSK108 library prep kit (protocol GDE_9002_v108_ revT_18Oct2016) and R9.4 chemistry.
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