Abstract
ABSTRACTA tomato-infecting tomato mosaic virus (ToMV) isolate was detected in Zimbabwe using lateral flow kits and double-antibody sandwich enzyme-linked immunosorbent assay. Next-generation sequencing and de novo assembly were subsequently performed to determine its genome sequence. The ToMV genome of the Zimbabwe isolate is the second to be reported in Africa.
Highlights
A tomato-infecting tomato mosaic virus (ToMV) isolate was detected in Zimbabwe using lateral flow kits and double-antibody sandwich enzyme-linked immunosorbent assay
The Zimbabwe ToMV isolate was analyzed for recombination events using the RDP4 program [6]
The full-genome and coat protein (CP) sequences of the Zimbabwe ToMV isolate and other ToMV isolates retrieved from GenBank were subjected to maximum likelihood phylogenetic analysis using the best-fit model of evolution for each data set with 1,000 bootstrap replicates and tobacco mosaic virus (TMV) as the outgroup
Summary
A tomato-infecting tomato mosaic virus (ToMV) isolate was detected in Zimbabwe using lateral flow kits and double-antibody sandwich enzyme-linked immunosorbent assay. The Quick-RNA mini prep kit (Zymo Research, South Africa) was used to extract total RNA from one randomly selected ToMV-positive sample. The RNA was sequenced on an Illumina MiSeq platform at Inqaba Biotechnical Industries (Pty) Ltd. FastQC (https://www.bioinformatics.babraham.ac.uk/projects/fastqc) was used to assess the quality of the reads.
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