Abstract
ABSTRACTA novel RNA virus was detected in a fecal sample collected from the Antarctic fur seal (Arctocephalus gazella) in King George Island, Antarctica. The almost-complete genome sequence reveals two open reading frames and a dicistrovirus-like gene order.
Highlights
A novel RNA virus was detected in a fecal sample collected from the Antarctic fur seal (Arctocephalus gazella) in King George Island, Antarctica
Aiming at the investigation of novel viruses of the Antarctic fur seal (Arctocephalus gazella) of the South Shetland Islands, Antarctica, fecal samples were collected in the southern summer seasons of 2013/2014 and 2014/2015 near Bellingshausen Station on King George Island
One contig corresponded to the helicase gene region, two overlapping contigs each to the proteinase gene region and the capsid protein region, respectively, and one contig to the polymerase gene region
Summary
A novel RNA virus was detected in a fecal sample collected from the Antarctic fur seal (Arctocephalus gazella) in King George Island, Antarctica. Aiming at the investigation of novel viruses of the Antarctic fur seal (Arctocephalus gazella) of the South Shetland Islands, Antarctica, fecal samples were collected in the southern summer seasons of 2013/2014 and 2014/2015 near Bellingshausen Station on King George Island. RNA was extracted with the NucliSENS kit (BioMérieux Deutschland GmbH) using an approximately 100-mg stool. Fifty nanograms of RNA extracted from a single sample taken on 15 February 2015 was employed for library preparation using the TruSeq stranded mRNA kit following the manufacturer’s description, except for the following part: in order to address all RNA molecules, purification of poly(A)ϩ molecules was not applied.
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