Abstract
ABSTRACTA new human gammapapillomavirus (HPV_MTS2) genome was isolated and fully cloned from a skin swab. The L1 open reading frame of HPV_MTS2 was 79% and 80% identical to those of its closest relatives, HPV type 149 (species Gamma-7 of the genus Gammapapillomavirus) and HPV isolate Dysk2 (GenBank accession no. KX781281), respectively, thus qualifying it as a new HPV type.
Highlights
A new human gammapapillomavirus (HPV_MTS2) genome was isolated and fully cloned from a skin swab
The amplified product was digested with EcoRI and cloned into the pUC19 vector for sequencing using a primer-walking strategy (GATC-Biotech, Germany), which covered nucleotides 647 to 6489 (5,843 bp) of the viral genome
To obtain the missing part of the viral genome, long-range PCR was performed on the rolling circle amplification (RCA) product template using TaKaRa LA Taq DNA polymerase and HPV_MTS2-specific primers
Summary
A new human gammapapillomavirus (HPV_MTS2) genome was isolated and fully cloned from a skin swab. The complete genome of a new HPV type (HPV_MTS2; 7,319 bp) was obtained by amplifying DNA from a human forearm skin swab using multiply-primed rolling circle amplification (RCA) according to the manufacturer’s instructions (illustra TempliPhi 100 amplification kit, GE Healthcare, USA). The amplified product was digested with EcoRI and cloned into the pUC19 vector for sequencing using a primer-walking strategy (GATC-Biotech, Germany), which covered nucleotides 647 to 6489 (5,843 bp) of the viral genome.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
