Abstract
ABSTRACTOceanospirillum multiglobuliferum ATCC 33336T is a motile gammaproteobacterium with bipolar tufted flagella, noted for its low salt tolerance compared to other marine spirilla. This strain was originally isolated from the putrid infusions of Crassostrea gigas near Hiroshima, Japan. This paper presents a draft genome sequence for O. multiglobuliferum ATCC 33336T.
Highlights
Oceanospirillum multiglobuliferum ATCC 33336T is a motile gammaproteobacterium with bipolar tufted flagella, noted for its low salt tolerance compared to other marine spirilla
O. multiglobuliferum was obtained from ATCC 33336T in freeze-dried form, rehydrated, and grown in marine peptone broth at 30°C for 72 h at 1 atm
After annotation with the NCBI Prokaryotic Genome Annotation Pipeline (PGAP) process [8], the assembly had a total length of 3,728,987 bp and an average coverage of 378ϫ
Summary
Oceanospirillum multiglobuliferum ATCC 33336T is a motile gammaproteobacterium with bipolar tufted flagella, noted for its low salt tolerance compared to other marine spirilla. O. multiglobuliferum is a halophile but has the lowest tolerance for salt in Oceanospirillum, growing in 0.5% to 4% (wt/vol) NaCl-containing peptone medium [1]. O. multiglobuliferum was obtained from ATCC 33336T in freeze-dried form, rehydrated, and grown in marine peptone broth at 30°C for 72 h at 1 atm. Isolated colonies on marine peptone agar plates were picked and grown in marine peptone broth, and the Genomic-tip 500/G kit (Qiagen, Valencia, CA, USA) was used to prepare genomic DNA (gDNA) from the culture.
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