Abstract
ABSTRACTThe vaccinia virus is a large, complex virus belonging to the Poxviridae family. Here, we report the complete, annotated genome sequence of the neurovirulent Western Reserve laboratory strain of this virus, which was sequenced on the Pacific Biosciences RS II and Oxford Nanopore MinION platforms.
Highlights
The vaccinia virus is a large, complex virus belonging to the Poxviridae family
The poly(A) fraction of the purified RNA was converted to cDNA following the Isoform Sequencing (Iso-Seq) protocol for Pacific Biosciences (PacBio) sequencing and the cDNA-seq protocol (1D strand switching cDNA by ligation) for the MinION process
The PacBio sequencing resulted in 59,154 reads, while 16,175 reads were obtained from the MinION runs
Summary
The vaccinia virus is a large, complex virus belonging to the Poxviridae family. Here, we report the complete, annotated genome sequence of the neurovirulent Western Reserve laboratory strain of this virus, which was sequenced on the Pacific Biosciences RS II and Oxford Nanopore MinION platforms. The CV-1 cell line (ATCC CCL-70) was infected with the laboratory WR strain of VV. The poly(A) fraction of the purified RNA was converted to cDNA following the Isoform Sequencing (Iso-Seq) protocol for PacBio sequencing and the cDNA-seq protocol (1D strand switching cDNA by ligation) for the MinION process. Seventeen single-molecule real-time (SMRT) cells with P6-C4 chemistry, as well as two MinION flow cells, were run on the PacBio and ONT systems, respectively. The PacBio sequencing resulted in 59,154 reads, while 16,175 reads were obtained from the MinION runs.
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