Abstract
Hibiscus rosa-sinensis is one of the most prevalent ornamental plants grown in private and public gardens. Hibiscus chlorotic ringspot virus (HCRSV) is a member of the Carmovirus genus, with a positive single-strand RNA that putatively encodes seven proteins. The complete genome of the first Israeli isolate of HCRSV, HCRSV-IL, comprises 3,908 nucleotides and shows 93% nucleotide sequence identity to the Singapore isolate and 87% identity to the Taiwanese isolate.
Highlights
Hibiscus rosa-sinensis is one of the most prevalent ornamental plants grown in private and public gardens
Since 2010, we have identified unfamiliar symptoms on hibiscus plants growing in public gardens across Israel
RNA was extracted from the virions and served as a template for a reverse transcription (RT) reaction, first with random hexamer and oligo(dT17) primers and later with the sequence-specific reverse complement primers R-HC-424 (5=TGTCAACCAACCTCCTTTCC-3=) and R-HC-1785 (5=-AAACA CCGGCTTCATTTGAC-3=)
Summary
Hibiscus rosa-sinensis is one of the most prevalent ornamental plants grown in private and public gardens. Since 2010, we have identified unfamiliar symptoms on hibiscus plants growing in public gardens across Israel. The disease symptoms include chlorotic spots, yellow rings, and vein banding. Symptomatic leafs were collected and used as a source material for viral purification as described previously (1).
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