Abstract
By utilising TLC with a developing system of petroleum ether-diethylether-acetic acid ( 70:30:2 v/v) and allowing the front to travel 18 cm, the best conditions for the fractionation of Bolti tissue lipids were achieved. Four distinct classes—triglycerides, free fatty acids, sterols and phospholipids—were detected. In addition, two other faint classes—hydrocarbons and diglycerides—were identified. Silica gel impregnated with silver nitrate has been employed for the fractionation of the triglycerides into groups. Seven groups were found when 1·5% of ethanol was added to the developing system of chloroform-acetic acid ( 99·5:0·5 v/v). Raising the ethanol content to 2% resulted in the detection of eight groups.
Published Version
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