Abstract
Summary: Protoplasts were formed from aged germlings and mycelia of Conidiobolus lamprauges, using a combination of chitinase and β-glucanase. Methanol, ethanol and propanol stimulated protoplast formation, apparently by a mechanism different from that of mercaptoethanol. Regeneration of protoplasts in soft agar was detectable by light microscopy 2 h after incubation and colonies were observable by the naked eye within 40 h; the regeneration rate was 65%.
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