Altered Antibiotic Production After Protoplast Preparation and Regeneration in Streptomyces Tenebrarius Mutants

Abstract

ABSTRACTThe techniques of protoplast preparation, regeneration and fusion gamed an important position in the strain improvement programs. They are successfully applied to obtain monoproducing strains from parent organisms synthesizing a group of related compounds. Protoplasling and protoplast regeneration in S. tenebrarius led to increased variability of the antibiotic biosynthesis, not only in the level of quantity, but in the quality of the products synthesized. Protoplast formation was observed in dependence on the age of the mycelium used for lysis, time of action of the lysozyme and finally the type of the strain. We determined the percentage of protoplasts able to resynthesize the cell wall and to reverte to mycelial growth on medium containing acceptable osmotic stabilization means. The revertants were checked for stability of morphology and antibiotic production. The effect of significant autoinhibition of regenerated protoplasts was observed. An increase in the morphological variability, connecte...