Abstract
Protoplast technique is a powerful tool for strain improvement and the preparation of protoplasts with high regeneration efficiency is the basis of subsequent manipulations. In this study, the effects of enzymatic hydrolysis time on release and regeneration of protoplasts from Aspergillus oryzae HN3042 were investigated by analyzing the time-series image. The results showed that there was a significant correlationship between regeneration efficiency and morphological size of protoplasts (P < 0.01). An efficient method for monitoring and improving the regeneration efficiency of protoplast was thus established by controlling the enzymatic hydrolysis process and the results can be referred to other species during the preparation and regeneration of protoplasts.
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