Abstract
The nearly complete genome sequence of a new species of potyvirus was obtained from the symptomless wild onion (Allium sp.) in Turkey. This virus has less than 67% nucleotide sequence identities over the polyprotein to other known potyviruses. We propose the name wild onion symptomless virus for this novel potyvirus.
Highlights
The nearly complete genome sequence of a new species of potyvirus was obtained from the symptomless wild onion (Allium sp.) in Turkey
turnip mosaic virus (TuMV), Japanese yam mosaic virus (JYMV), narcissus late season yellows virus (NLSYV), narcissus yellow stripe virus (NYSV), and scallion mosaic virus (ScaMV) are species included in the TuMV group in the potyvirus phylogeny
RT-PCR products of approximately 2,100 bp from the nuclear inclusion b protein gene to the 3=-end poly(A) [NIb-poly(A)] region were amplified with potyvirus-specific primer pairs modified from the primers described by Zheng et al [7] and Ohshima et al [1], POTYNIbNOT4P (5=-GGGGCGGCCGCATATGGGGTGAGA GAGGTNTGYGTNGAYGAYTTYAAYAA-3=) and Tu3T9 M (5=-GGGGCGGCCGCT15-3=)
Summary
The nearly complete genome sequence of a new species of potyvirus was obtained from the symptomless wild onion (Allium sp.) in Turkey. One aim of our recent studies is to find new viral species within the group of viruses related to turnip mosaic virus (TuMV) [1] to assess evolutionary relationships between the viruses. TuMV, Japanese yam mosaic virus (JYMV), narcissus late season yellows virus (NLSYV), narcissus yellow stripe virus (NYSV), and scallion mosaic virus (ScaMV) are species included in the TuMV group in the potyvirus phylogeny.
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