First Genome Sequence of Shallot Latent Carlavirus from Allium macrostemon Bunge.

Yasuhiro Tomitaka,Kazuo Yamashita,Kanji Ishimaru,Ryosuke Yasaka,Kazusato Ohshima,Kouta Okamura,Shinji Fukuda

doi:10.1128/genomea.00809-17

Abstract

ABSTRACTA wild Japanese garlic plant (Allium macrostemon Bunge, wild onion) with leaves showing chlorotic stripes was collected in Saitama Prefecture, Japan. Genome sequencing showed that it was infected with shallot latent carlavirus. The genomic sequence of this virus is reported for the first time from wild onion.

Highlights

A wild Japanese garlic plant (Allium macrostemon Bunge, wild onion) with leaves showing chlorotic stripes was collected in Saitama Prefecture, Japan
A part of the Shallot latent virus (SLV) genome was identified by RT-PCR when SLV-specific primer pairs with a NotI restriction site were used
These primer pairs amplify the region from the middle of the TGB1 coding region to the 3= end of SLV genome

Summary

Introduction

A wild Japanese garlic plant (Allium macrostemon Bunge, wild onion) with leaves showing chlorotic stripes was collected in Saitama Prefecture, Japan. Sap from specimen WOST87 was manually inoculated to leaves of Chenopodium quinoa test plants, and chlorotic local lesions appeared on them. Viruses known to infect Allium spp. and to cause local lesions in C. quinoa were cucumber mosaic cucumovirus, tomato spotted wilt tospovirus, tobacco mosaic tobamovirus, and shallot latent carlavirus. Tests using enzyme-linked immunosorbent assays or reverse transcription-PCR (RT-PCR) (PrimeScript II high-fidelity one-step RT-PCR kit; Takara Bio, Shiga, Japan) were used to identify the virus.

Results

Conclusion