Abstract
ABSTRACTCorynebacterium diphtheriae has caused multiple isolated diphtheria cases in Malaysia over the years. Here, we report the first draft genome sequences of 15 Malaysia C. diphtheriae clinical isolates collected from the years 1981 to 2016.
Highlights
Corynebacterium diphtheriae has caused multiple isolated diphtheria cases in Malaysia over the years
The 15 isolates of C. diphtheriae were confirmed following the method shown by Efstratiou et al (2)
The sequencing was performed using a whole-genome shotgun approach performed on an Illumina MiSeq system (Illumina, San Diego, USA) with 2 ϫ 301 bp paired-end chemistry
Summary
Corynebacterium diphtheriae has caused multiple isolated diphtheria cases in Malaysia over the years. The 15 isolates of C. diphtheriae were confirmed following the method shown by Efstratiou et al (2). The genomic DNA was extracted using a Gram-positive DNA purification kit (Epicentre, Madison, WI, USA) following the manufacturer’s protocol and a Nextera XT DNA sample preparation kit (Illumina, San Diego, CA, USA) was used to construct DNA libraries.
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