First Complete Squash leaf curl China virus Genomic Segment DNA-A Sequence from East Timor.

Solomon Maina,Abel Ximenes,Roger A C Jones,Owain R Edwards,Luis De Almeida

doi:10.1128/genomea.00483-17

Abstract

ABSTRACTWe present here the first complete Squash leaf curl China virus (SLCCV) genomic segment DNA-A sequence from East Timor. It was isolated from a pumpkin plant. When compared with 15 complete SLCCV DNA-A genome sequences from other world regions, it most resembled the Malaysian isolate MC1 sequence.

Highlights

We present here the first complete Squash leaf curl China virus (SLCCV) genomic segment DNA-A sequence from East Timor
The 15 East Timorese samples were blotted onto fast technology for analysis of nucleic acids (FTA) cards [19] before dispatch to Australia
Total RNA was extracted from both sample types using a ZR Plant RNA MiniPrep kit (Zymo Research)

Summary

Introduction

We present here the first complete Squash leaf curl China virus (SLCCV) genomic segment DNA-A sequence from East Timor. The 15 East Timorese samples were blotted onto fast technology for analysis of nucleic acids (FTA) cards [19] before dispatch to Australia. The Australian samples studied were recently collected leaves. Total RNA was extracted from both sample types using a ZR Plant RNA MiniPrep kit (Zymo Research).

Results

Conclusion