Abstract
Micrococcus sp. strain V7, an actinobacterial strain adapted to the extreme conditions of the Laguna Vilama, an extremely high-altitude (4,600 m above sea level) lake in the Argentinian Puna, was found to carry the giant linear plasmid pLMV7. We determined its sequence (92,815 bp) as a prerequisite to the investigation of its role in survival in such a harsh environment.
Highlights
High-altitude lakes in the Argentinian Puna are extreme and pristine environments, but organisms living in such habitats must adapt to very harsh conditions, such as high UV radiation, oligothrophy, and a high arsenic concentration
DNA analysis by pulsed-field gel electrophoresis disclosed the presence of a 92-kb linear extrachromosomal genetic element, which has terminal proteins covalently attached to the 5=-ends; the linear plasmid was termed pLMV7 [3]
As the host adaptation traits might be at least partially conferred by pLMV7, the plasmid was fully sequenced, being to the best of our knowledge the first accessory linear genetic element that has been completely sequenced in the genus Micrococcus
Summary
High-altitude lakes in the Argentinian Puna are extreme and pristine environments, but organisms living in such habitats must adapt to very harsh conditions, such as high UV radiation, oligothrophy, and a high arsenic concentration. DNA analysis by pulsed-field gel electrophoresis disclosed the presence of a 92-kb linear extrachromosomal genetic element, which has terminal proteins covalently attached to the 5=-ends; the linear plasmid was termed pLMV7 [3]. As the host adaptation traits might be at least partially conferred by pLMV7, the plasmid was fully sequenced, being to the best of our knowledge the first accessory linear genetic element that has been completely sequenced in the genus Micrococcus. Purified plasmid was sequenced by combining Sanger sequencing and 454 pyrosequencing.
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