Abstract
We report here the first complete sequences of two Keystone virus (KEYV) genomes isolated from Florida in 2005, which include the first two publicly available complete large (L) gene sequences. The sequences of the KEYV L segments show 75.99 to 83.86% nucleotide similarity with those of other viruses in the California (CAL) serogroup of bunyaviruses.
Highlights
Keystone virus (KEYV) belongs to the California (CAL) serogroup of arboviruses in the family Bunyaviridae [1]
The KEYVs were amplified in Vero cells, and viral RNA was extracted from cell culture supernatant using TRIzol and chloroform
De novo contigs were searched against the GenBank nonredundant nucleotide database (NT) using BLASTN, which identified the longest contigs with high similarity matches to the KEYV M and S segments
Summary
Keystone virus (KEYV) belongs to the California (CAL) serogroup of arboviruses in the family Bunyaviridae [1]. Bunyaviruses are enveloped viruses with negative-sense single-stranded RNA (ssRNA) and tripartite genomes consisting of large (L), medium (M), and small (S) RNA segments [3]. This study generated the first two complete genome sequences for the KEYV isolated in 2005 from the Tampa Bay area, FL.
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