Abstract
ABSTRACTIn this study, we determined for the first time the complete genomic sequence of an Argentinian isolate of Potato leafroll virus (PLRV), the type species of the genus Polerovirus. The isolate sequenced came from a Solanum tuberosum plant that had been naturally infected with the virus. Isolate PLRV-AR had a nucleotide sequence identity between 94.4 and 97.3% with several known PLRV isolates worldwide.
Highlights
In this study, we determined for the first time the complete genomic sequence of an Argentinian isolate of Potato leafroll virus (PLRV), the type species of the genus Polerovirus
The ORF2 sequence of a PLRV isolate, which was detected in 1999, is the only partial sequence reported of an Argentinian PLRV isolate [5]
Total RNA was isolated from infected leaf samples using the TriPure isolation reagent (Roche Molecular Systems, Inc., USA), and cDNA was synthesized with reverse transcriptase SuperScript III (Thermo Fisher Scientific, Inc., USA)
Summary
We determined for the first time the complete genomic sequence of an Argentinian isolate of Potato leafroll virus (PLRV), the type species of the genus Polerovirus. The ORF2 sequence of a PLRV isolate, which was detected in 1999, is the only partial sequence reported of an Argentinian PLRV isolate [5]. To obtain a complete genome sequence of an Argentinian isolate of PLRV, we obtained a field-grown infected S. tuberosum cv. Total RNA was isolated from infected leaf samples using the TriPure isolation reagent (Roche Molecular Systems, Inc., USA), and cDNA was synthesized with reverse transcriptase SuperScript III (Thermo Fisher Scientific, Inc., USA).
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
