Abstract
E-mail: hoeil@hanyang.ac.krReceived October 22, 2010, Accepted December 26, 2010We have determined the urea concentration in an aqueous solution using Raman spectroscopy by incorporatinga Teflon tube as an effective intensity correction standard as well as sample container. A non-overlappingTeflon band was used as the reference peak to correct Raman intensity variations that occasionally resultedfrom changes in laser power. To increase the sensitivity, we positioned a copper reflector inside the Teflon tubeto maximize the collection of Raman scattering. The obtained accuracy using Raman spectroscopy was 0.53mM, close to the range of accuracy of previous NIR studies (0.15-0.52 mM).Key Words : Urea, Hemodialysis, Intensity correction, Copper reflector, Raman spectroscopyIntroductionWith the use of NIR (near-infrared) spectroscopy, themonitoring of urea concentration during hemodialysis hasbeen recently studied.
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