Abstract
Objective To study the expression of Wnt5a gene mRNA and protein in human hep-atocellular carcinoma(HCC) and explore its clinical significance. Methods Wnt5a mRNA level was measured in 22 patients with HCC and paired non-tumorous liver tissues by real-time RT-PCR. Immu-nohistochemical staining of Wnt5a was performed in 76 patients with HCC using ABC system. The re-lationship between expression of Wnt5a gene and clinicopathological parameters in patients with HCC was analyzed. Results The Wnt5a was overexpressed in 15 of 22 hepatocellular carcinomas (68%). Wnt5a was highly expressed in hepatocellular carcinoma cells in contrast to its low level of expression in non-neoplastic liver cells(P=0.038). The reduced Wnt-5a protein expression, evident in 80.3% (61/76) of the HCCs, was significantly associated with higher serum AFP level(P=0.020) and poor tumor stage(P 0.05). Conclusion The cytoplasmic loss of Wnt-5a protein is a frequent progression event in HCC. Our data support the notion that Wnt-Sa retains tumor suppressor function and may be a useful indicator of poor prognosis in HCC. Key words: Carcinoma, hepatocellular; Wnt5a; Real-time RT-PCR; Immunohistochemistry
Published Version
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