Abstract
Objective To explore the role of miR-145 in human hepatocellular carcinoma ( HCC). We investigate the expression of miR-145 in human embryonic hepatocytes ( L02 cells),hepatocellular carcinoma cell lines (MHCC97 ,SMMC7721) ,HCC tissues and normal liver. Methods We detected the expressions of miR-145 in human embryonic hepatocytes, hepatocellular carcinoma cell lines ( MHCC97,SMMC7721) ,HCC tissues,cancer organizations and normal liver by real-time PCR. Result The expression of miR-145 in hepatocellular carcinoma cell lines ( MHCC97,SMMC7721) is significantly lower than the human embryonic hepatocytes ( 0. 312 ± 0. 025,0. 396 ± 0. 076,0. 954 ± 0. 037, P ＜ 0. 05), and compared with the cancer organizations and normal liver,the expression of miR-145 in HCC tissues is significantly decreased (0. 162 ±0.002,0. 972 ±0.054,0.956 ±0. 018,P＜0. 05). Conclusion These results indicate that the miR-145 probably involved in HCC pathogenesis and was the indicator of diagnosis and prognosis of the HCC. Key words: miR-145; HCC; Real-time PCR
Published Version
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