Abstract
Objective To explore the expression of protease activated receptor 3 (PAR3) and protease activated receptor 4 (PAR4) in the progression from colorectal polyps to cancer. Methods In this study, five groups of specimens were researched: primary colorectal cancer group (n=30), matched normal colorectal tissues group (taken 5 cm away from the tumour) (n=30), tubular adenoma group (n=30), villous adenoma group (n=20), and adenoma mixed group (n=30). The protein and mRNA levels of PAR3 and PAR4 were detected by immunohistochemistry and real-time fluorescent quantitative polymerase chain reaction (FQ-PCR) respectively. Results The positive expression rate of PAR3 protein in normal colorectal mucosa, colorectal adenoma and colorectal carcinoma tissues was 73.3%, 50.0% and 6.7% respectively, with significant differences among them (P=0.009). The positive expression rate of PAR4 protein in these groups was 6.7%, 35.0% and 70.0% respectively. FQ-PCR showed that the expression of PAR3 mRNA in normal colorectal mucosa, tubular adenoma, mixed pattern of adenoma, villous adenoma and colorectal carcinoma was 0.787±0.040, 0.453±0.023, 0.410±0.050, 0.368±0.032 and 0.259±0.017, P=0.011; and that of PAR4 mRNA in these groups was 0.370±0.301, 10.384±1.474, 20.892±4.485, 36.311±7.953, 52.083±12.550 (F=43.342, P=0.009, respectively). Conclusion These results suggested PAR3 and PAR4 may play a role in the development of colorectal cancer. Key words: Protease activated receptor 3; Protease activated receptor 4; Colorectal cancer; Colorectal adenoma
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call