Experimental Researches Regarding in vitro Multiplication Innovation by Direct Organogenesis in Brussels Cabbage (Brassica oleracea L. convar. oleracea, var. gemmifera)

Abstract

Modern biotechnologies offer the possibility to multiplication the biologic vegetal structures by shaping the morphological phenomena (organogenesis or embryogenesis), which ensure both the maintenance of their characteristic elements as well as their multiplication in a number of units as extended as possible, starting with a certain type of explant and its intrinsic capacity, according to which, under the influence of in vitro culture, certain cells pertaining to it manifest properties specific to stem vegetal cells. In consequence, the aim of the experimental researches presented in this study was the innovation of in vitro multiplication at Brussels cabbage ( Brassica oleracea L. convar. oleracea var. gemmifera ), from subvarieties Rosella and Sanda , by direct caulogenesis induced under the influence of explant type (leaf, cotyledon, epicotyl, hypocotyl and radicle) and through direct rhizogenesis induced under the influence of exogenous auxin type (NAA or IBA). The best caulogenesis response in case of each of the two subvarieties of Brussels cabbage ( Brassica oleracea L. convar. oleracea var. gemmifera ) was recorded by experimental variant that consisted of using the explants of cotyledon type. While the best direct rhizogenesis response at Rosella subvariety of Brussels cabbage ( Brassica oleracea L. convar. oleracea var. gemmifera ) was recorded on experimental variant that consisted of using NAA exogenous auxin 0.25 mg/l, and in case of Sanda subvariety of Brussels cabbage ( Brassica oleracea L. convar. oleracea var. gemmifera ) the best direct rhizogenesis response was recorded on experimental variant that consisted of using IBA exogenous auxin 0.75 mg/l.