Experimental research on in vitro propagation through direct somatic embryogenesis of basil (Ocimum basilicum L.).

Abstract

Induction, generation and development of embryogenic somatic phenomena are matters that can be modeled by monitoring the interplay between exogenous phytohormones and explants. As a result, this work presents experimental studies that illustrate the influence that exogenous cytokinins (BAP and TDZ), and auxins (NAA or IBA) have, added in the composition of the artificial nutritive medium, for modeling the in vitro propagation through direct somatic embryogenesis in two cultivars of basil (Ocimum basilicum L. var. Marseille and Ocimum basilicum L. var. Red Rubin), starting from different types of explants (leaf, cotyledon, epicotyl, hipocotyl and radicle). The best embryogenic response was recorded for experimental variant consisting in TDZ cytokinin and IBA auxin in quantities of 1 mg/l respectively 0,5 mg/l, and cotyledon type explant.