Study Regarding In Vitro Propagation through Somatic Embryogenesis in Beta vulgaris L.

Abstract

Abstract. In vitro propagation through somatic embryogenesis constitutes one the main concerns of the current vegetal biotechnologies. The regeneration system of vitroplants by means of somatic embryogenesis eliminates their sequential regeneration, because it allows the simultaneous induction and materialization both of the root pole and of the radicular pole [8]. The use of exogenous phytohormones in modeling the morphogenetic processes performed through cultures of cells and tissue in plants constitutes a renowned practice in the specialty literature. In the case of somatic embryogenesis, the specialists recommend the sequential use of cytokinins and auxins. Considering the above information and taking into account the fact that the profitability of an in vitro multiplication protocol depends mainly on the shortening of the growing period, we studied the unitary involvement of the ratio between auxins and cytokinins as a primordial element in achieving the states of induction, competence and determination for directing somatic embryogenesis in the red beet (Beta vulgaris subsp. vulgaris var. vulgaris Red Ace and Beta vulgaris subsp. vulgaris var. vulgaris Cylindra). The best embryogenetic answer was recorded for the experimental variant that combined BAP cytokinine and ANA auxine, in quantities of 0.5 mg/l respectively 0.2 mg/l, and the cotyledon type explant.