Study regarding in vitro multiplication in Begonia sp.

Abstract

The in vitro multiplication of plants that have commercial value constitutes one of the major objectives of the present vegetal biotechnologies. In the in vitro propagation process achieved on plants. the use of exogene phytohormones constitutes a decisive element for the feasibility of their multiplication (Cachi?? – Cosma Dorina. C. Deliu. Lenu?a Rakosy – Tican. A. Ardelean. 2004). The inducing and development. of adventitious caulogenetic and rhizogenetic phenomena. are aspects that can be materialized by the use of adequate combinations between cytokinin and auxin. Also. a well known fact is that different types of explants contribute to the achievement of the in vitro culture with an endogenous content in phytohormones. As a result. this study presents the influence the exogenous cytokinins (TDZ. BA) and auxins (IAA. NAA) added to the composition of the Murashige & Skoog (1962) nutritive medium in the modeling of the in vitro propagation process in three cultivars of Begonia sp. starting from different types of explants (single-node stem cuttings with axillary buds. internode. leaf. peduncle. flower in the stage of bud). The materialization of caulogenesis had the best response at the level of the explants of the flower in the stage of bud type. by using the experimental variant that combines the TDZ (1.0 mg/l) and BA (0.5 mg/l) cytokinins.Rhizogenesis registered maximum values in case of the experimental variant that benefited from the IAA and NAA auxins. in quantities of 0.5 mg/l each. It must also be noticed the fact that rhizogenesis was achieved and in the absence of an exogenous auxinic source.