Experimental research of Hedyotic diffusa injection on the inhibition of the proliferation of human osteosarcoma cell line MG-63 in vitro

Abstract

Objective To investigate the inhibition of Hedyotic diffusa injection on the proliferation of human osteosarcoma cell line MG-63 in vitro. Methods The human osteosarcoma cell line MG-63 was subcultivated aseptically in vitro.Different concentration of Hedyotic diffusa injection(50μL/mL,100μL/mL,200μL/mL,400μL/mL)successively acted on such cell.A total three time points were selected to determine the activity and numbers of cells by MTT assay including 12h,24h and 48h. Results The cellular proliferation inhibition rates of human osteosarcoma cell line MG-63 in drug groups of 50μL/mL concentration holes were(2.87±2.22)%,(13.42±2.14)% and(30.80±3.67)% after 12 h,24 h and 48 h.The rates of 100μL/mL concentration holes were(22.25±1.58)%,(43.34±2.84)% and(66.46±2.64)%,after 12 h,24 h and 48 h.The rates gradually increased and had statistical meaning,t12h= 12.319,t24h= 14.573,t48h = 12.319,P<0.05;the cell in drug groups of 200μL/ml and 400μL/ml concentration holes was totally dead,and pathological findings showed that there were circular and floating cells in which nucleoplasmic ratio decreased and small fragments of cells increased. Conclusion Hedyotic diffusa extract has a certain inhibition in vitro on the proliferation of human osteosarcoma cell line MG-63.Moreover,the strength of its inhibition is relevant probably with drug concentration, which deserves a further research. Key words: Human osteosarcoma cell line MG-63; Cell proliferation; Hedyotic diffusa extract