Inhibitory effects of curcumin on growth of human osteosarcoma cell line MG-63 cells

Abstract

Objective To investigate the inhibitory effects of curcumin (Cur) on proliferation and apopotosis of human osteosarcoma cell line MG-63 cells.Methods The cultured MG-63 cells were treated with Cur (12.5,25.0,50.0,100.0 μmol/L) for 12 h.The morphologic changes of MG-63 cells were observed by inverted microscope.The survival rate of MG-63 cells was measured by using methyl thiazol tetrazolium (MTT) assay.Cell counting of kit-8 (CCK-8) assay was used to test the growth inhibitory rate.Annexin V/propidium iodide (PI) staining was applied to detect the apoptosis.The survival ratios and the adhesion capacity of MG-63 were detected except the lactate dehydrogenase (LDH) releasing.Results Different concentrations of Cur (12.5,25.0,50.0,100.0 μmol/L) observed in this study have significant effects on the survival ratio of MG-63.The Cur could significantly inhibit the growth of MG-63 in a dose and time dependent manner and induce G2/M Phase cell cycle arrest.After 12 h,cell inhibition rate of 47.23％,24 h after the cell was 65.23％.Cur 12.5,25.0,50.0,100.0 μmol/L group for 24 h,MG-63 cell apoptosis rate was (11.24 ± 0.07)％,(28.23 ± 0.35)％,(50.21 ± 1.71)％ and (41.22 ±1.85) ％,50.0 μmol/L curcumin could induce apoptosis obviously.Conclusion When Cur increased from 12.5 μmol/L to 25.0 μmol/L,its MG-63 cells at 24h growth inhibition rate (13.56 ± 1.88) ％ to (48.27 ±2.32)％,50.0 μmol/L group 24 h growth inhibition rate to 65.23％.50.0 μmol/L of Cur on the MG-63 cells apoptosis induced by the most obvious,for 24 h,apoptosis rate was (50.21 ± 1.71)％. Key words: Curcumin ; Osteosarcoma; Proliferation