Abstract
Bacteria are ubiquitous in nature, live on animate and inanimate surfaces playing diverse roles and act as either beneficial or harmful organisms. The present study focuses on developing a simple and cost-effective method for long-term storage of methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-susceptible Staphylococcus aureus (MSSA). The method involves drying bacterial cultures viz., nine MRSA strains and one MSSA strain, on gelatin-impregnated filter paper strips. A 12% nutrient gelatin medium was used for maintaining and storing the bacterial cultures. The results showed that the bacteria preserved on the gelatin-paper strips remained viable for up to a year at temperatures of 4°C, -20°C, or -80°C.Further, the bacterial cultures survived for over a month at room temperature (30±2 °C). This practical, user-friendly technique offers an affordable solution to store and transport bacterial cultures, making it particularly beneficial for labs with limited resources or storage space.
Published Version
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