Abstract
Background: Every year, many people around the world die from cancers. Among all types of cancers, brain cancer has been recognized as one of the most deadly cancers due to the late detection and limitations of current therapies, and thus it remains an unresolved problem. Glioblastoma occurs in different parts of the central nervous system and is one of the most important causes of cancer death in people. In addition, there are many problems for the treatment of cancer cells. One of the limiting factors is the resistance of cancer cells to chemotherapy drugs. In this regard, the use of nanoparticles (NPs) is an effective method for overcoming this problem. Materials and Methods: In this study, iron oxide-NPs were synthesized and loaded on the folic and lomustine. Further, the size and morphology of NPs were assessed by transmission electron microscopy, X-ray photoelectron spectroscopy, and dynamic light scattering. Then, the U87-MG cell line was cultured in the Dulbecco’s Modified Eagle Medium and treated with nano, nano-folic, nano-lomustine (LUM), LUM, and complex, followed by evaluating 50% inhibitory concentration, tetrazolium assay, and caspase-6 activity. Results: Our results showed that cell viability decreased in LUM container groups by increasing the incubation time. Based on the caspase-6 activity analysis, the mortality rate increased in LUM container groups after 3 days. These findings indicated that LUM, complex, and nano-LUM increase cell death in U87MG. Conclusion: Finally, the results suggested that LUM in NPs could be applied as a safer form of drug delivery for targeting cancer.
Highlights
Cancer is recognized as one principal cause of mortality worldwide
Glioblastoma occurs in different parts of the central nervous system and is considered as the main leading cause of cancer death in people under 35 years of age [6, 7]
One of the limiting factors is the resistance of cancer cells to chemotherapy drugs and the low doses of these drugs next to cancerous cells because of the side effects, early renal and liver excretion, as well as the presence of a blood-brain barrier [10, 11]
Summary
Cancer is recognized as one principal cause of mortality worldwide. A large number of people around the world die from cancers every year. Creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited This may increase the survival of patients after surgery, the prognosis of this cancer is still not convincing. One of the limiting factors is the resistance of cancer cells to chemotherapy drugs and the low doses of these drugs next to cancerous cells because of the side effects, early renal and liver excretion, as well as the presence of a blood-brain barrier [10, 11]. These limiting factors should be eliminated to achieve the desired result. Given the above-mentioned explanations, the present study evaluated and compared the effect of nano, nanofolic, nano-LUM, LUM, and complex on cell viability and apoptosis in U87MG brain tumor cells
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
